Question 1 · Medium
To take a wound swab for culture using the Levine technique, the nurse first cleans the wound with normal saline and then:
- ARolls the swab across the whole wound in a zigzag line without pressure
- BDips the swab into the pus and slough collected on the dressing
- CWipes the swab around the wound edge and the surrounding skin
- DRotates the swab with pressure over 1 cm2 of clean wound bed
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Answer: D · Rotates the swab with pressure over 1 cm2 of clean wound bed
The Levine technique samples the organisms in the tissue rather than on the surface. Loose exudate and debris are first rinsed away with saline so that surface colonisers are not cultured. The swab is then pressed and rotated over about 1 cm2 of viable tissue for about 5 seconds, until fluid is drawn up. Dressing contents, edges and slough mostly grow colonising bacteria.
- A: A light zigzag over the surface without pressure samples surface colonisers.
- B: Old exudate on the dressing grows colonisers, not the organism in the tissue.
- C: The edge and skin carry skin flora, which give a misleading result.