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Physical examination: color, clarity, specific gravity, osmolality

15 ASCP MLS practice questions on Physical examination: color, clarity, specific gravity, osmolality, part of Urinalysis and Body Fluids. The first 15 are below; the first 5 with answers and explanations (tap Show answer after you have chosen), the rest in Learn with a free account.

  1. Question 1 · Hard

    At 11:00, the routine refractometer check gives: deionized water 1.003; 5% sodium chloride 1.025 (expected 1.022 ± 0.001); 9% sucrose 1.037 (expected 1.034 ± 0.001). The 07:00 check was in range, and 18 patient urines have been read on this instrument since then, one of them at 1.038 with negative glucose and protein and no recent imaging. The prism and cover were cleaned and dried before the check, the room is at 23 °C, and the refractometer is temperature-compensated from 15 °C to 38 °C. What should the technologist do?

    1. ARelease the results, as the error is trivial
    2. BSubtract 0.003 from each result and release them
    3. CRezero with water, repeat QC, then retest the urines
    4. DCorrect each reading for the room temperature
    Show answer

    Answer: C · Rezero with water, repeat QC, then retest the urines

    Every reading, including water, which should read 1.000, is 0.003 too high, so the error is a constant offset across the scale: a zero error. It is corrected by adjusting the zero set screw with deionized water and then rechecking the 5% NaCl and 9% sucrose controls. Because both controls failed, the urines read since the last acceptable check must be retested once QC is back in range. Patient results are never adjusted by hand to make up for a failed control. A reading of 1.038, above the upper reference value of 1.035, with no glucose, protein or contrast medium to explain it, was itself a clue to the problem.

    • A: Both controls are outside their ±0.001 limits, so results since the last good check cannot be released.
    • B: Results are not corrected by hand; the instrument is fixed and the specimens are retested under acceptable QC.
    • D: The refractometer is temperature-compensated across this range, so no temperature correction applies.
  2. Question 2 · Medium

    Every urine a patient passes over 24 hours has a specific gravity close to 1.010, whatever the fluid intake. This pattern is most consistent with:

    1. AOsmotic diuresis from glucosuria
    2. BA normal response to high fluid intake
    3. CCentral diabetes insipidus (no ADH)
    4. DLoss of tubular concentrating ability
    Show answer

    Answer: D · Loss of tubular concentrating ability

    Glomerular filtrate has a specific gravity of about 1.010. Urine that stays fixed at this value (isosthenuria) shows that the tubules can no longer concentrate or dilute the filtrate, as in advanced chronic kidney disease. Urine below 1.010 is hyposthenuric and urine above it is hypersthenuric. A single random value of 1.010 means little; a fixed value across specimens is what matters.

    • A: Glucosuria raises the specific gravity, giving high values with polyuria.
    • B: Healthy kidneys dilute urine well below 1.010 after a fluid load.
    • C: Diabetes insipidus gives persistently dilute urine, well below 1.010.
  3. Question 3 · Easy

    During refractometer quality control, what should 5% sodium chloride read?

    1. A1.010 ± 0.001
    2. B1.034 ± 0.001
    3. C1.022 ± 0.001
    4. D1.000 ± 0.001
    Show answer

    Answer: C · 1.022 ± 0.001

    The refractometer is first set to read 1.000 with distilled water, using the zero set screw if needed. The calibration is then checked with a solution of known specific gravity: 5% sodium chloride should read 1.022 ± 0.001, or 9% sucrose 1.034 ± 0.001. If a check is out of range, clean the prism and recheck before testing patient urines. Results are recorded as part of daily quality control.

    • A: 1.010 is the specific gravity of glomerular filtrate, not a quality control standard.
    • B: 1.034 ± 0.001 is the expected reading for 9% sucrose.
    • D: 1.000 is the zero reading for distilled water.
  4. Question 4 · Medium

    A refrigerated urine with pH 5.5 has a pink, brick-dust precipitate that dissolves when the tube is warmed. The pink color comes from:

    1. AUroerythrin
    2. BPorphobilin
    3. CMyoglobin
    4. DUrobilin
    Show answer

    Answer: A · Uroerythrin

    Cold, acidic urine precipitates amorphous urates, and the normal pink pigment uroerythrin attaches to them. This gives the typical pink brick-dust sediment. Amorphous urates dissolve when warmed to about 60 °C, while amorphous phosphates in alkaline urine are white and dissolve in dilute acetic acid instead. Keeping routine specimens at room temperature when they are tested within 2 hours avoids this precipitate.

    • B: Porphobilin gives a red to port-wine color in porphyria, not a pink sediment.
    • C: Myoglobin is dissolved in the urine and does not precipitate in the cold.
    • D: Urobilin tints the whole specimen orange-brown as urobilinogen oxidizes; it is not bound to a precipitate.
  5. Question 5 · Hard

    A 30-year-old woman has severe abdominal pain, weakness and confusion. Her fresh urine looked normal, but after hours on the bench in daylight it has turned port-wine red. The strip is negative for blood, and no red cells are seen. The color is most likely due to:

    1. ABetalain pigment from eating beets
    2. BPorphobilin from porphobilinogen
    3. CHomogentisic acid from alkaptonuria
    4. DMyoglobin from damaged muscle
    Show answer

    Answer: B · Porphobilin from porphobilinogen

    In acute intermittent porphyria, a deficiency of porphobilinogen deaminase (hydroxymethylbilane synthase) causes excess porphobilinogen and aminolevulinic acid, with attacks of abdominal pain and neurologic symptoms. Porphobilinogen is colorless, but on exposure to air and light it oxidizes to porphobilin, which is red to port-wine. The blood pad is negative because no heme pigment is present. Protect the urine from light and test it promptly for porphobilinogen.

    • A: Beet pigment colors urine red soon after eating beets; it does not develop on standing or cause these symptoms.
    • C: Homogentisic acid turns urine black, not red, and alkaptonuria does not cause acute attacks.
    • D: Myoglobin is a heme protein, so it would make the blood pad positive.
  6. Question 6 · Medium

    Why is osmolality a better measure of renal concentrating ability than specific gravity?

    1. AIt depends only on the number of solute particles
    2. BIt is not affected by glucose or urea in the urine
    3. CIt gives more weight to large solute molecules
    4. DIt measures only the sodium and chloride ions

    Answer and explanation in LearnFree account

  7. Question 7 · Medium

    A cloudy red urine is centrifuged. The supernatant is clear and yellow, with a red button at the bottom of the tube. What does this indicate?

    1. AFree hemoglobin (hemoglobinuria)
    2. BFree myoglobin (myoglobinuria)
    3. CPorphyrins (porphyrinuria)
    4. DIntact red cells (hematuria)

    Answer and explanation in LearnFree account

  8. Question 8 · Easy

    A dark amber urine forms yellow foam when shaken. Which substance does this suggest?

    1. AProtein
    2. BUrochrome
    3. CBilirubin
    4. DUrobilin

    Answer and explanation in LearnFree account

  9. Question 9 · Hard

    A urine reads 1.032 on a refractometer. It contains glucose 2,000 mg/dL (20 g/L) and protein 1,000 mg/dL (10 g/L). What is the specific gravity corrected for glucose and protein?

    1. A1.043
    2. B1.022
    3. C1.021
    4. D1.024

    Answer and explanation in LearnFree account

  10. Question 10 · Medium

    An elderly woman with a long-term urinary catheter and chronic constipation has purple urine in her collection bag. Urine pH is 8.0, and culture grows Providencia stuartii. The purple color is most likely due to:

    1. AMelanin from melanogen
    2. BIndigo and indirubin from indoxyl sulfate
    3. CPorphobilin from porphobilinogen
    4. DMethemoglobin from hemoglobin

    Answer and explanation in LearnFree account

  11. Question 11 · Hard

    Two hours after a CT scan with intravenous contrast, a patient's urine gives: refractometer SG 1.052, reagent strip SG 1.015, pH 6.0, glucose and protein negative. Refractometer QC with water and 5% NaCl is in range. The best explanation is:

    1. AThe refractometer's zero setting has drifted
    2. BSevere dehydration has concentrated the urine
    3. CAlkaline pH is lowering the strip reading
    4. DContrast medium is raising the refractometer reading

    Answer and explanation in LearnFree account

  12. Question 12 · Medium

    A woman being treated for a painful urinary tract infection brings in thick, bright orange urine that forms orange foam when shaken. Which drug is most likely responsible?

    1. ARifampin
    2. BMethylene blue
    3. CPhenazopyridine
    4. DNitrofurantoin

    Answer and explanation in LearnFree account

  13. Question 13 · Easy

    A refractometer measures urine specific gravity by measuring:

    1. ABending of light passing through the sample
    2. BChange in pKa of a polyelectrolyte
    3. CChange in frequency of a sound wave
    4. DDepression of the sample's freezing point

    Answer and explanation in LearnFree account

  14. Question 14 · Easy

    Which pigment gives normal urine most of its yellow color?

    1. AUroerythrin
    2. BUrobilin
    3. CUrochrome
    4. DBilirubin

    Answer and explanation in LearnFree account

  15. Question 15 · Easy

    Newsprint viewed through a well-mixed urine is blurred but still visible. How should the clarity be reported?

    1. ACloudy
    2. BTurbid
    3. CHazy
    4. DMilky

    Answer and explanation in LearnFree account

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